Effects of Morphine and Electroacupuncture on Substance P Level in Spinal Cord and Their Relation to Pain Threshold in Rats / 第三军医大学学报

Immunoreactive substance P (Ir-SP) level and substance P-like activity (SP-LI)in the spinal cord were determined with radioimmunoassay and immunohistochemistry in rats after they were given an intraperitoneal injection of morphine(7.5mg/kg)or electroacupunctured(3V and 3Hz)on the "Jiaji" point. It was found that pain threshold,Ir-SP content,and SP-LI activity in the dorsal horn of the spinal cord were significantly increased in the experimental group than in the control (P

Potentiation Effect of Diazepam on ACTH Analgesia in the Mice / 第三军医大学学报

Experimental methodssintraperitoneal injection of diazepam,r-aminobutyric acid (GABA) receptor antagonist (picrotoxin) and GABA synthesis inhibitor (iso-niazid) ;the pain threshold and serotonin ( 5 -HT) content in the brain are measured with spectrofluorometry.The effect of diazepam on ACTH analgesia (AA) is abstracted and the possible mechanisms of diazepam effect are studied.Results: diazepam increases the effect of AA.picroloxin and isoniazid reduce it and antagonize the analgesia effect of ACTH; diazepam increases the content of 5-HT in hipocampus,hypothalamus and midbrain;diazepam + ACTH increases,more than AC-TH or diazepam singly,the level of 5-HT in the 3 brain regions; picrotoxin and isoniazid reverse the effect of diazepam and ACTH on the content of 5-HT in the 3 brainsites; potentiation of diazepam on AA is antagonized by naloxone and atropine.